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酵母对 金头海鲷先天性免疫的影响

VeterinaryImmunologyandImmunopathology85(2002)41±50

Oraladministrationofyeast,Saccharomycescerevisiae,enhancesthecellularinnateimmuneresponseofgiltheadseabream(SparusaurataL.)

Âguez,ÂsOrtunÄo,AlbertoCuesta,AlejandroRodrõJesu

ÂMeseguer*M.AngelesEsteban,Jose

FacultyofBiology,DepartmentofCellBiology,UniversityofMurcia,30071Murcia,SpainReceived29June2001;receivedinrevisedform16October2001;accepted16October2001

Abstract

Theeffectsofincludinglyophilisedwholeyeast,Saccharomycescerevisiae,inthedietontheseabreaminnateimmune

responsewereinvestigated.Giltheadseabream(SparusaurataL.)specimenswerefedfourdifferentdietsfor4weeks:acommercialdietascontrolandthesamedietsupplementedwith1,5or10g/kgyeast.After1,2and4weeks,serumcomplementtitres,asahumoralparameter,andphagocytic,respiratoryburst,myeloperoxidaseandnaturalcytotoxicactivitiesofhead-kidneyleucocytes,ascellularparameters,wereevaluated.Theresultsshowedthatyeastsupplementsenhancedallthelatterresponses,butnotthehumoralresponse.Thisenhancementwasdose-dependentexceptforthecytotoxicactivitythatwasonlystimulatedbythelowerdoseofyeastassayed.Asyeastcellwallsareabletoenhancetheseabreamcellularinnateimmuneresponse,theseresultssupportthepossibleuseofwholeyeastasnaturalinmunostimulantsincommon®shdiets.#2002ElsevierScienceB.V.Allrightsreserved.

Keywords:Yeast;Saccharomycescerevisiae;Diet;Immunomodulation;Innateimmunesystem;Leucocytes;Giltheadseabream(SparusaurataL.)

1.Introduction

Naturalimmunostimulantsarevaluableforactivat-ingthe®shimmunesystemandprotecting®shagainstadverseconditions(Anderson,1992;Sakai,1999).Amongthem,yeastSaccharomycescerevisiaehasbeenfoundtobeagoodenhancerofthetroutimmunesystem(Siwickietal.,1994).Yeastcellwallsareconstructedalmostentirelyoftwoclassesof

Correspondingauthor.Tel.: 34-68-364-965;fax: 34-968-363-963.

E-mailaddress:meseguer@um.es(J.Meseguer).

*

polysaccharides,mannosepolymerscovalentlylinkedtopeptides(ormannoprotein)andglucosepolymers(orglucans).Theglucansandmannoproteinsoccurinroughlyequalamountsinthewall.AthirdsugarpolymerofN-acetylglucosamine,chitin,isalsoanimportantcomponentoffungalwalls,butispresentonlyinminoramounts(about1%)inyeasts(CabibandRoberts,1982).b-Glucansandchitinhavebeendescribedaspowerfulimmunostimulantsin®shandmammals,whiletheuseofyeastmannoproteinsasimmunostimulantshaveonlybeenstudiedinmam-mals(Vecchiarelli,2000).b-Glucanshavegenerallybeendemonstratedtoactivate®shmacrophagesinvitroandtoenhancethenon-speci®cdefenceof®sh

酵母对 金头海鲷先天性免疫的影响

酵母对 金头海鲷先天性免疫的影响

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0165-2427/02/$±seefrontmatter#2002ElsevierScienceB.V.Allrightsreserved.PII:S0165-2427(01)00406-8

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